2B4 anticorps (AA 20-374)

N° du produit ABIN7600551

Cet anticorps Lapin Polyclonal détecte spécifiquement 2B4 dans WB et ELISA. Il présente une réactivité envers Souris.

Aperçu rapide pour 2B4 anticorps (AA 20-374) (ABIN7600551)

Antigène: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp 2B4 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-2B4 anticorps

Épitope: AA 20-374

Fonction: Anti-2B4/Cd244 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-2B4/Cd244 Antibody Picoband® (ABIN7600551). Tested in ELISA, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived mouse 2B4/Cd244 recombinant protein (Position: Q20-N374).

Isotype: IgG

Information d'application

Indications d'application: "Western blot, 0.25-0.5 μg/mL, Mouse
ELISA, 0.1-0.5 μg/mL, -
"1. Boles, K. S., Nakajima, H., Colonna, M., Chuang, S. S., Stepp, S. E., Bennett, M., Kumar, V., Mathew, P. A. Molecular characterization of a novel human natural killer cell receptor homologous to mouse 2B4. Tissue Antigens 54: 27-34, 1999. 2. Brown, M. H., Boles, K., van der Merwe, P. A., Kumar, V., Mathew, P. A., Barclay, A. N. 2B4, the natural killer and T cell immunoglobulin superfamily surface protein, is a ligand for CD48. J. Exp. Med. 188: 2083-2093, 1998. 3. Suzuki, A., Yamada, R., Kochi, Y., Sawada, T., Okada, Y., Matsuda, K., Kamatani, Y., Mori, M., Shimane, K., Hirabayashi, Y., Takahashi, A., Tsunoda, T., Miyatake, A., Kubo, M., Kamatani, N., Nakamura, Y., Yamamoto, K. Functional SNPs in CD244 increase the risk of rheumatoid arthritis in a Japanese population. Nature Genet. 40: 1224-1229, 2008. 4. Tangye, S. G., Lazetic, S., Woollatt, E., Sutherland, G. R., Lanier, L. L., Phillips, J. H. Cutting edge: human 2B4, an activating NK cell receptor, recruits the protein tyrosine phosphatase SHP-2 and the adaptor signaling protein SAP. J. Immun. 162: 6981-6985, 1999. 5. Watzl, C., Stebbins, C. C., Long, E. O. Cutting edge: NK cell inhibitory receptors prevent tyrosine phosphorylation of the activation receptor 2B4 (CD244). J. Immun. 165: 3545-3548, 2000.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur 2B4

Antigène: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Autre désignation: Cd244

Sujet:

Synonyms: Natural killer cell receptor 2B4, NK cell type I receptor protein 2B4, NKR2B4, Non-MHC restricted killing associated, SLAM family, SLAMF4, Signaling lymphocytic activation molecule 4, CD244, Cd244, 2b4, Nmrk

Tissue Specificity: Expressed in natural killer (NK) cells, T cells and dendritic cells.

Background: CD244 (Cluster of Differentiation 244) is a human protein encoded by the CD244 gene. It is also known as Natural Killer Cell Receptor 2B4. Tangye et al. (1999) mapped the 2B4 gene to 1q22. Suzuki et al. (2008) identified a functional single-nucleotide polymorphism (SNP) in the CD244 gene that contributes to rheumatoid arthritis susceptibility. Functional analysis by Boles et al. (1999) demonstrated that engagement of 2B4 with specific antibody activates NK cytolytic activity. Using recombinant human NK cell-activating ligand 2B4 fused to domains 3 and 4 of rodent Cd4 and flow cytometric analysis, Brown et al. (1998) demonstrated that CD48 binds to 2B4. Watzl et al. (2000) showed that antibody-mediated cross-linking of 2B4 leads to its rapid tyrosine phosphorylation, which is necessary for 2B4-mediated killer cell activity.

Poids moléculaire: 50 kDa

ID gène: 102927459

UniProt: Q07763